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目的:检测上皮性卵巢癌组织原发灶、转移灶中miR-7及其靶标EGFR蛋白的表达,探讨miR-7及EGFR与卵巢癌转移的关系。方法:采用显色原位杂交(CISH)检测miR-7在卵巢癌组织原发灶、转移灶石蜡切片中的表达;采用免疫组化检测EGFR蛋白表达。结果:上皮性卵巢癌转移灶中miR-7表达显著低于原发灶(P<0.05),而EGFR表达显著高于原发灶(P<0.05);miR-7与EGFR表达具有相关性(r=-0.441,P<0.05)。结论:miR-7可能通过EGFR抑制卵巢癌的转移,miR-7可能成为治疗卵巢癌的新靶标。
OBJECTIVE: To detect the expression of miR-7 and its target EGFR protein in epithelial ovarian cancer tissues and metastases, and to explore the relationship between miR-7 and EGFR and the metastasis of ovarian cancer. Methods: The expression of miR-7 in primary and metastatic paraffin sections of ovarian cancer was detected by colorimetric in situ hybridization (CISH). The expression of EGFR protein was detected by immunohistochemistry. Results: The expression of miR-7 in epithelial ovarian cancer was significantly lower than that in primary tumor (P <0.05), while the expression of EGFR was significantly higher than that in primary tumor (P <0.05). The expression of miR-7 was correlated with EGFR r = -0.441, P <0.05). Conclusion: miR-7 may inhibit the metastasis of ovarian cancer through EGFR, and miR-7 may be a new target for the treatment of ovarian cancer.