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目的采用nPCR技术,对来我院确诊的非淋球菌性尿道炎(NGU)患者泌尿生殖道分泌物标本进行检测,以探讨套式PCR(nPCR)技术在生殖支原体(Mg)和解脲脲原体(Uu)检测中的作用和地位。方法选择深圳市福田区NGU患者98例,宫颈或尿道口内拭子分泌物标本用nPCR进行DNA扩增,并与对照组43例进行比较。结果98例标本中Mg阳性15例,阳性率为15.3%,正常对照组无一例阳性。Uu阳性21例,阳性率为21.4%,对照组仅1例阳性。结论深圳福田区Mg、Uu在NGU中具有较高的感染率,nPCR具有敏感、快速、特异性强等特点,可用于临床辅助诊断和流行病学调查。
Objective To detect genitourinary secretions secreted by non-gonococcal urethritis (NGU) patients diagnosed in our hospital by nPCR technique in order to investigate the effects of nested PCR (nPCR) technique on genital mycoplasma (Mg) and ureaplasma urealyticum (Uu) test in the role and status. Methods 98 patients with NGU in Futian District of Shenzhen City were selected. Nasal or urethral swab secretions samples were amplified by nPCR and compared with 43 cases of control group. Results Among the 98 specimens, 15 were positive for Mg, the positive rate was 15.3%. No positive result was found in the normal control group. Uu positive in 21 cases, the positive rate was 21.4%, only 1 positive in the control group. Conclusion Mg and Uu in Futian district of Shenzhen have a high infection rate in NGU. NPCR is sensitive, rapid and specific and can be used in clinical diagnosis and epidemiological investigation.