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膳食纤维在人体中的发酵程度对其生理作用有很大的影响。盲肠是细菌发酵膳食纤维的重要部位,大鼠的盲肠较大,为了评价大鼠实验模型本文比较了人和大鼠肠道中膳食纤维的降解及其增加粪便体积的能力。膳食纤维由干燥的麦麸、苹果、包菜及胡萝卜分别用甲醇液(850 ml甲醇/1000 ml水)提取3小时,过滤后再用丙酮提取3小时,自然干燥,磨成粉过0.5~1.4 mm筛孔制得。瓜儿豆胶(guar gum)为市售品。用1M H_2SO_4水解非纤维性多糖,其不溶解的残渣作为纤维素,经12 M H_2SO_4水解后用气-液色谱法测
The degree of fermentation of dietary fiber in the human body has a great influence on its physiological role. The cecum is an important part of bacterial fermentation of dietary fiber and the caecum of rats is larger. In order to evaluate the experimental model in rats, we compared the degradation of dietary fiber in human and rat intestines and their ability to increase fecal volume. The dietary fiber was extracted from dried wheat bran, apple, cabbage and carrot for 3 hours with methanol solution (850 ml methanol / 1000 ml water respectively), filtered and then extracted with acetone for 3 hours, dried naturally and ground to a powder score of 0.5 to 1.4 mm sieve obtained. Guar gum is commercially available. With 1M H_2SO_4 hydrolysis of non-fibrous polysaccharides, the insoluble residue as cellulose, 12 M H_2SO_4 after hydrolysis by gas-liquid chromatography