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目的建立乌药生物碱提取物中总生物碱含量测定的方法。方法采用酸性染料比色法测定乌药生物碱提取物中总生物碱含量。结果对照品去甲异波尔定的线性范围为4.444～44.44 g/ml,线性方程为Y=0.03785C+0.04626(r=0.9995),平均加样回收率为98.19%,RSD为1.64%(n=9)。结论本法操作简便、准确,可用于乌药生物碱提取物中总碱含量的测定。
Objective To establish a method for the determination of total alkaloids in the alkaloid extract of Radix Aconiti Lateralis. Methods The total alkaloid content of alkaloids extract of Radix Astragali was determined by acid dye colorimetry. Results The linear range of norvalopidine was 4.444 ~ 44.44 g / ml and the linear equation was Y = 0.03785C + 0.04626 (r = 0.9995). The average recovery was 98.19% and the RSD was 1.64% (n = 9). Conclusion This method is simple, accurate and can be used for the determination of total alkaloids in the extract of alkaloids of Radix.