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目的:研究SD大鼠两种胫骨骨缺损模型构建的差异,为建立标准、有效骨缺损模型提供参考。方法:将60只SD大鼠随机分为克氏针组、双层钢锯组,克氏针组用低速电钻造成直径为1mm的圆孔单皮质骨缺损(此为钻孔法);双层钢锯组用钢锯造成宽度为1mm、深至见髓腔出血的骨缺损(此法为锯开法)。对两组平均造模耗时、术后肿胀情况、造模成功率、畸形愈合率、行走站立活动评分进行比较。结果:克氏针组各项观察指标均优于双层钢锯组,且术后24h肿胀程度及术后1周、2周行走站立活动评分差异具有统计学意义(P<0.05)。结论:钻孔法和锯开法均可成功构建胫骨骨缺损模型,钻孔法操作简便、高效且创伤小,是理想的胫骨缺损模型制备方法。
OBJECTIVE: To study the differences of two kinds of tibial defect models in SD rats and to provide a reference for establishing standard and effective bone defect models. Methods: Sixty Sprague-Dawley rats were randomly divided into Kirschner wire group, double-layer hacksaw group, Kirschner wire group with a diameter of 1mm single hole cortical defect with a diameter of 1mm (this is the drilling method) A hacksaw group with a hacksaw width of 1mm, deep bone marrow bleeding see the bone defect (this method is sawing method). The mean modeling time, postoperative swelling, the success rate of modeling, the rate of malunion and walking standing scores were compared. Results: The Kirschner wire group was better than the double-hacksaw group in observation indexes, and the degree of swelling at 24 hours after operation and the scores of standing and walking activities at 1 week and 2 weeks after operation were statistically significant (P <0.05). CONCLUSION: Both the drilling method and the sawing method can successfully construct the tibial bone defect model. The drilling method is simple, efficient and less invasive and is an ideal method for the preparation of the tibial defect model.