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白细胞介素－８（ＩＬ－８）是一种分布广泛的细胞因子，但其在肝癌细胞的定位尚未见报道。本研究采用免疫组织化学ＡＢＣ－ＧＤＮ法，对ＩＬ－８在培养的大鼠肝癌ＦＳＫ７９０２细胞系的分布进行了定位。结果显示，ＩＬ－８样免疫反应阳性物质分布于所有肝癌细胞，阳性物质为棕黑色颗粒状，颗粒均匀一致，主要集中分布于胞核周围的胞质中。ＩＬ－８样免疫反应阳性物质在肝癌细胞的分布并不完全一致，在未形成细胞单层的有突起的癌细胞中，ＩＬ－８样免疫反应阳性物质主要存在于胞核周围的胞质中，细胞突起的胞质中无ＩＬ－８样免疫反应阳性物质。本实验证明，肝癌细胞在不需要细胞因子刺激的情况下，可自发地表达ＩＬ－８（组成性表达）。
Interleukin-8 (IL-8) is a widely distributed cytokine, but its location in liver cancer cells has not been reported yet. In this study, immunohistochemical ABC-GDN method was used to locate the distribution of IL-8 in cultured rat hepatocarcinoma FSK7902 cell line. The results showed that IL-8-like immunoreactive substances distributed in all liver cancer cells, the positive substance is brown black granular, uniform particles, mainly concentrated in the cytoplasm around the nucleus. The distribution of IL-8-like immunoreactive substances in hepatoma cells is not exactly the same. IL-8-like immunoreactive substances mainly exist in the cytoplasm around the nucleus in the cells with protuberances that do not form monolayers , There was no IL-8-like immunoreactive substance in the cytoplasm of the cell protrusion. This experiment demonstrates that hepatoma cells spontaneously express IL-8 (constitutive expression) without the need for cytokine stimulation.