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目的:探讨大鼠肠缺血再灌注(IR)时p38 MAPK特异性抑制剂SB203580对p38 MAPK、凋亡调控因子及凋亡细胞表达的影响。方法:Wistar大鼠30只随机分为对照组(C组)、缺血再灌注组(Ⅰ组)和SB203580组(S组)(n=10),采用肠IR模型检测p38 MAPK、Bcl-2、Bax、TNF-及凋亡指数的水平。结果:Ⅰ组中p38 MAPK、凋亡调控因子及凋亡细胞的表达均显著高于C组(P<0.05),而S组中p38 MAPK、TNF-α及凋亡细胞的表达减少,Bcl-2/Bax比值增高(P<0.05)。结论:SB203580抑制了小肠组织中p38 MAPK通路的活化,从而增加了Bcl-2的表达、减少了Bax和TNF-α的释放,在缓解细胞凋亡的过程中起到了重要的作用。
OBJECTIVE: To investigate the effect of SB203580, a specific inhibitor of p38 MAPK, on the expression of p38 MAPK, apoptotic regulatory factors, and apoptotic cells in rat intestinal ischemia-reperfusion (IR). Methods: Thirty Wistar rats were randomly divided into control group (C group), ischemia-reperfusion group (group I) and SB203580 group (S group) (n=10). The intestinal IR model was used to detect p38 MAPK and Bcl-2. , Bax, TNF-, and the level of apoptosis index. Results: The expressions of p38 MAPK, apoptotic regulatory factors and apoptotic cells in group I were significantly higher than those in group C (P<0.05), while the expression of p38 MAPK, TNF-α and apoptotic cells were decreased in S group. The ratio of 2/Bax increased (P<0.05). CONCLUSIONS: SB203580 inhibits the activation of the p38 MAPK pathway in the small intestine, thereby increasing the expression of Bcl-2, reducing the release of Bax and TNF-α, and plays an important role in relieving apoptosis.