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目的:观察山楂叶总黄酮对棕榈酸损伤的胰岛βTC3细胞是否具有保护作用,并筛查出山楂叶总黄酮所起作用的有效浓度。方法:以胰岛βTC3细胞为研究对象,使用棕榈酸制作脂毒性模型,采用MTT法观察山楂叶总黄酮是否具有保护作用,并进一步筛查出有效浓度及时间;同时采用末端脱氧核苷酸转移酶介导的dUTP原位切口末端标记(TUNEL)技术检测胰岛βTC3细胞的凋亡情况。结果:MTT结果显示10-100μg/ml的山楂叶总黄酮对棕榈酸损伤的胰岛βTC3细胞均有保护作用,其吸光度值明显比棕榈酸组高(P<0.05),并且50μg/ml的山楂叶总黄酮与棕榈酸共同处理胰岛βTC3细胞24小时具有最好的保护作用;TUNEL检测结果显示山楂叶总黄酮+棕榈酸组胰岛βTC3细胞的凋亡率比棕榈酸组低(P<0.01)。结论:山楂叶总黄酮对脂毒性损伤的胰岛βTC3细胞具有保护作用,并在10-100μg/ml浓度范围内成一定的剂量依赖效应。
Objective: To observe whether hawthorn leaves flavonoids have protective effect on palmitate-damaged islet βTC3 cells and screen the effective concentration of hawthorn leaf flavonoids. Methods: Pancreatic βTC3 cells were used as the research object. The model of lipotoxicity was made by using palmitic acid. The MTT method was used to observe whether the total flavonoids of hawthorn leaf had the protective effect and the effective concentration and time were further screened. Meanwhile, terminal deoxynucleotidyl transferase Mediated dUTP nick end labeling (TUNEL) technique was used to detect the apoptosis of islet βTC3 cells. Results: MTT assay showed that the total flavonoids of hawthorn leaves with 10-100μg / ml had protective effect on the islet βTC3 cells damaged by palmitate, the absorbance values were significantly higher than that of palmitic acid group (P <0.05), and hawthorn leaves of 50μg / ml Total flavonoids and palmitic acid co-treated islet βTC3 cells for 24 hours with the best protective effect; TUNEL test results showed that hawthorn leaf flavonoids + palmitic acid group βTC3 cells apoptosis rate was lower than the palmitic acid group (P <0.01). Conclusion: The total flavonoids of hawthorn leaves have a protective effect on the islet βTC3 cells damaged by lipotoxicity, and have dose-dependent effect in the range of 10-100μg / ml.