采用免疫组化染色检测３０ 例皮肤鳞癌组织细胞周期蛋白Ｄ１（ｃｙｃｌｉｎ Ｄ１） 、细胞周期蛋白依赖性激酶４（ＣＤＫ４） ，ｐ１６ 及Ｒｂ 的表达，阳性率依次为６６－７ ％ ，５３－３ ％ ，３３－３ ％ 和３６－７ ％ ；ｃｙｃｌｉｎ Ｄ１ 及ＣＤＫ４ 于癌巢边缘细胞表达强于中央癌细胞，且其分化程度越低，阳性着色细胞越多，着色越深。提示细胞周期尤其Ｇ１ 期的正向调节因子的过度表达及负向调节因子的低表达在皮肤鳞状细胞癌的发生、发展中起重要作用。 Immunohistochemical staining was used to detect the expression of cyclin D1, cyclin dependent kinase 4 (CDK4), p16 and Rb in 30 cases of cutaneous squamous cell carcinoma. The positive rate was 66-7 %, 53-3. % ,33-3 % and 36-7 % ; cyclin D1 and CDK4 expressed more strongly in cancer nests than in central cancer cells , and the lower the degree of differentiation ,the more positive staining cells were, the deeper the coloration was. It suggests that overexpression of positive regulators of cell cycle, especially G1 phase, and low expression of negative regulatory factors play an important role in the occurrence and development of cutaneous squamous cell carcinoma.