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目的探讨单核细胞趋化蛋白-1(MCP-1)和巨噬细胞炎性蛋白-2(MIP-2)在急性胰腺炎(AP)早期发病中的作用,观察褪黑素(MT)对趋化因子MCP-1及MIP-2表达的影响。方法35只SD大鼠随机分为假手术(SO)组,AP 3、6、12 h组和MT 3、6、12 h干预组。采用4%牛磺胆酸钠胰胆管逆行注射制备AP动物模型,MT组在诱导AP前0.5 h给予腹腔注射MT 20 mg/kg。观察胰腺病理组织学改变,采用实时定量RT-PCR方法检测各组胰腺组织中MCP-1 mRNA、MIP-2 mRNA表达水平。结果AP大鼠胰腺组织中MCP-1 mRNA、MIP-2 mRNA表达显著高于SO组(P<0.01),且表达水平与胰腺病理严重程度成正相关(P<0.05)。经MT干预后,胰腺组织中MCP-1 mRNA、MIP-2 mRNA表达下调(P<0.05),胰腺组织损伤得到改善。结论MCP-1及MIP-2在AP大鼠发病过程中具有重要作用,MT可能通过下调其在胰腺组织的表达,对AP具有一定的保护作用。
Objective To investigate the role of monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-2 (MIP-2) in the pathogenesis of early acute pancreatitis (AP). To observe the effects of melatonin Effects of chemokines MCP-1 and MIP-2 expression. Methods Thirty-five Sprague-Dawley rats were randomly divided into sham operation (SO) group, AP 3, 6 and 12 h groups and MT 3, 6 and 12 h intervention groups. The animal model of AP was prepared by retrograde injection of 4% sodium taurocholate and cholangiopancreatic duct. The MT group was intraperitoneally injected with MT 20 mg / kg 0.5 h before induction of AP. The histopathological changes of pancreas were observed. The expression of MCP-1 mRNA and MIP-2 mRNA in pancreatic tissue were detected by real-time quantitative RT-PCR. Results The expression of MCP-1 mRNA and MIP-2 mRNA in pancreatic tissues of AP rats was significantly higher than that of SO rats (P <0.01). The expression of MCP-1 mRNA and MIP-2 mRNA was positively correlated with the severity of pancreatic lesions (P <0.05). After MT intervention, the expression of MCP-1 mRNA and MIP-2 mRNA in pancreatic tissue was down-regulated (P <0.05), and the pancreatic tissue injury was improved. Conclusions MCP-1 and MIP-2 play an important role in the pathogenesis of AP rats. MT may protect APs by down-regulating their expression in pancreatic tissues.