论文部分内容阅读
目的在基因转录水平了解12条细胞表面受体介导的信号通路相关基因对大鼠肝再生的调控作用。方法用搜集网站资料和查阅相关论文等方法获得上述基因,用大鼠基因组230 2.0芯片检测它们在大鼠再生肝中的表达情况,用比较手术和假手术中基因的表达差异性确定肝再生相关基因。结果初步证实上述基因中491个与肝再生相关,其中226个基因之间有742种直接作用关系。细胞因子和趋化因子介导的酶联受体、G蛋白耦联受体、谷氨酸;抗原受体介导的,整合素介导的,脂多糖介导的,Notch、Osmosensory、Smoothened、Toll、Wnt等12条信号通路中与肝再生相关的上调基因数和下调基因数,分别为26和23、164和54、59和51、5和1、22和14、21和10、1和1、4和11、23和11、32和17。肝再生中基因上调表达次数和下调表达次数分别为188和128、464和190、308和207、13和5、88和46、123和50、2和1、20和43、148和30、174和62。结论除Osmosensory和脂多糖介导的信号通路在肝再生中作用较小,Smoothened信号通路作用减弱外,其他9条信号通路在肝再生中作用增强。
Objective To understand the regulatory effect of 12 cell surface receptor-mediated signal transduction related genes on rat liver regeneration at the gene transcriptional level. Methods The above genes were obtained by means of collecting website information and consulting relevant papers and so on. The rat genomic 230 2.0 was used to detect the expression of these genes in rat regenerative liver. The relative expression of genes in surgical and sham operation was used to determine the correlation between liver regeneration gene. Preliminary results confirmed that 491 of the above genes were associated with liver regeneration, of which there were 742 direct interactions between 226 genes. Cytokines and Chemokine-Mediated Enzyme Receptors, G-Protein Coupling Receptors, Glutamate; Antigen Receptor-Mediated, Integrin-Mediated, Lipopolysaccharide Mediated, Notch, Osmosensory, Smoothened, Toll, Wnt and other 12 signaling pathways related to liver regeneration up-regulated genes and down-regulated genes were 26 and 23,164 and 54,59 and 51,5 and 1,22 and 14,21 and 10,1 and 1, 4 and 11, 23 and 11, 32 and 17. The numbers of up-regulated and down-regulated genes in liver regeneration were 188 and 128, 464 and 190, 308 and 207, 13 and 5, 88 and 46, 123 and 50, 2 and 1, 20 and 43, 148 and 30, 174 And 62. Conclusion Osmosensory and lipopolysaccharide mediated signaling pathways play a minor role in liver regeneration, and the effect of Smoothened signaling pathway is attenuated. The other nine signaling pathways play an important role in liver regeneration.