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目的：研究Ｐ５３基因状况与抗癌化疗敏感性的关系，对抗癌药耐药机制进行探讨。方法：用ＭＴＴ法测定１７种抗癌药对８６例恶性肿瘤的抑制率，并用聚合酶链反应———单链构象多态分析技术检测其Ｐ５３基因状况。结果：统计比较抗癌药在Ｐ５３基因正常组和Ｐ５３基因异常组中的抑制率，发现多数抗癌药抑制率前者显著地大于后者。结论：当恶性肿瘤中存在Ｐ５３基因突变或缺失时，抗癌药耐药性显著增加。
Objective: To study the relationship between the status of P53 gene and the sensitivity of anti-cancer chemotherapy, and explore the mechanism of anti-cancer drug resistance. Methods: MTT assay was used to determine the inhibition rate of 17 anticancer drugs against 86 malignant tumors, and the status of P53 gene was detected by polymerase chain reaction-single strand conformation polymorphism analysis technique. RESULTS: Statistical comparison of the inhibition rates of anticancer drugs in the normal group and the abnormal group of P53 gene showed that the majority of the anticancer drug inhibitory rate was significantly greater than the latter. CONCLUSION: When there is a mutation or deletion of P53 gene in malignant tumors, the anti-cancer drug resistance increases significantly.