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A组轮状病毒是引起成人和婴幼儿急性腹泻的主要病原。了解轮状病毒流行株的型别,对主要中和抗原VP7的编码基因进行遗传变异分析,可为当地轮状病毒疫苗的应用和开发提供指导。我们对2012年10月至2013年12月南京地区908例腹泻门诊患者的粪便标本进行轮状病毒检测,采用RT-PCR方法对随机抽取的50份阳性标本进行G分型,并对其中19份G9型轮状病毒的VP7基因序列测序分析。结果发现轮状病毒阳性率11.34%(103/908),其中以G9型为主,占78.0%(39/50),其次是G2、G1和G3型。对G9型轮状病毒VP7基因核苷酸序列进行分析,显示主要分为G9-VI亚型和G9-III亚型,以G9-VI亚型为主,且属于中国和日本G9型轮状病毒亚簇,部分毒株在A、B、C、F四个中和抗原表位中有变异,这可能有助于G9型轮状病毒的流行,值得引起注意。
Group A rotavirus is the major cause of acute diarrhea in adults and infants. Understanding the types of rotavirus strains and analyzing the genetic variation of the coding gene of the major neutralizing antigen VP7 may provide guidance for the application and development of the local rotavirus vaccine. We detected rotavirus in 908 cases of diarrhea outpatients in Nanjing from October 2012 to December 2013. RT-PCR was used to genotype G in 50 randomly selected positive samples and 19 of them Sequence analysis of VP7 gene of G9 rotavirus. The positive rate of rotavirus was found to be 11.34% (103/908), of which G9 type accounted for 78.0% (39/50), followed by G2, G1 and G3. Analysis of the nucleotide sequence of VP7 gene of G9 rotavirus revealed that it was mainly divided into G9-VI subtype and G9-III subtype, G9-VI subtype, and belonged to China and Japan G9 rotavirus Sub-clusters, some of the strains in A, B, C, F four neutralization epitopes have variation, which may contribute to the epidemic of G9-type rotavirus, deserve attention.