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目的:建立右旋葡聚糖硫酸钠(DSS)诱导的大鼠溃疡性结肠炎模型,探索经直肠灌注Gd-nanoCPs检测炎性肠病的预临床方法。方法:采用改良的乳化凝结技术合成Gd-nanoCPs。采用5%葡聚糖硫酸钠诱导溃疡性结肠炎大鼠模型。对正常组(n=14)、结肠炎组(n=14)分别灌注Gd-nanoCPs混悬液(10只),Gd-DTPA水溶液(2只)及尾静脉注射Gd-DTPA(2只),于灌肠前和灌肠20～120min后行结肠MR扫描。对结肠上段、中段及下段肠壁分别进行灌肠前后结肠壁、盆壁肌肉和图像背景MR信号强度进行测量,计算灌肠前后肠壁与盆壁肌肉的相对信号值及强化率,并采用配对t检验进行组间比较。结果:直肠灌注Gd-nanoCPs混悬液20～120min内肠黏膜均有强化,20～120min内下段、中段及上段结肠黏膜强化率分别为1.8%～35.2%、1.6%～26.4%、1.8%～27.3%和25.2%～71.1%、20.8%～55.7%、9.4%～35.1%。两组差异有统计学意义(P<0.05)。结论:Gd-nanoCPs作为一种新型肠道MRI对比剂,具有成为检测炎性肠病的特异性靶向MRI造影剂的潜能。
OBJECTIVE: To establish a rat model of ulcerative colitis induced by dextran sodium sulfate (DSS) and to explore a pre-clinical method for detecting inflammatory bowel disease by rectal perfusion of Gd-nanoCPs. Methods: Gd-nanoCPs were synthesized by a modified emulsion coagulation technique. Induction of Ulcerative Colitis in Rats by 5% Dextran Sodium Sulfate. Gd-nanoCPs suspension (10), Gd-DTPA aqueous solution (2), and Gd-DTPA (2) were intravenously injected into the normal group (n = 14) and colitis group (n = 14) Before enema and enema 20 ~ 120min after colon MR scan. Before and after the enema, the signal intensity of the colon wall, the pelvic wall muscle and the image background were measured before and after the enema. The relative signal value and enhancement rate of the intestinal wall and the pelvic wall muscle before and after the enema were calculated. The paired t test Compare between groups. Results: The intestinal mucosa of Gd-nanoCPs suspension was enhanced within 20-120 min in rectum perfusion, and the rates of colon mucosal mucosa were 1.8% -35.2%, 1.6% -26.4%, 1.8% ~ 27.3% and 25.2% -71.1%, 20.8% -55.7% and 9.4% -35.1%, respectively. The difference between the two groups was statistically significant (P <0.05). CONCLUSION: Gd-nanoCPs, as a novel intestinal MRI contrast agent, has the potential to become a specific targeted MRI contrast agent for the detection of inflammatory bowel disease.