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目的:比较奈达铂(NDP)体外对人宫颈腺癌细胞Hela细胞及鳞癌细胞Siha的抑制作用并探讨其作用机制。方法:以2.5～40μg/ml NDP分别作用于Hela及Siha细胞24 h,48 h,72 h,MTT法检测抑制率,计算半数抑制浓度(IC50);利用流式细胞术(FCM)观察细胞凋亡率及周期变化;半定量PCR法分析基因半胱氨酸天门冬氨酸蛋白酶3(Caspase 3,Caspase 9)及基质金属蛋白酶2(MMP-2)的表达变化。结果:NDP对宫颈癌Hela细胞及Siha细胞均有抑制作用,呈时间-剂量依赖性,NDP对Hela细胞的抑制率大于Siha细胞;FCM显示,随时间、浓度增加,Hela及Siha细胞凋亡率增加,相同浓度和作用时间下,Hela细胞的凋亡率大于Siha细胞(P<0.05),S期细胞的比例增加,G0/G1期比例减少,G2/M期比例变化不明显;与对照组相比,Caspase 3,Caspase 9表达增加,MMP-2表达减少。结论:NDP可抑制宫颈腺癌细胞Hela及鳞癌细胞Si-ha的增殖,NDP体外对Hela细胞的抑制作用更强。NDP的作用机制与诱导细胞凋亡、细胞周期阻滞、上调Caspase3和Caspase9的表达有关;下调MMP-2的表达可能有利于降低宫颈癌细胞的侵袭力。
OBJECTIVE: To compare the inhibitory effect of NDP on Si-a human cervical adenocarcinoma cell line Hela and squamous cell carcinoma Siha and to explore its mechanism. METHODS: Hela and Siha cells were treated with 2.5 ~ 40μg / ml NDP for 24 h, 48 h, 72 h, respectively. The inhibition rate was measured by MTT assay and the IC50 value was calculated. Flow cytometry (FCM) The changes of caspase 3, Caspase 9 and MMP-2 were analyzed by semi-quantitative PCR. Results: NDP inhibited Hela cells and Siha cells in a time-and dose-dependent manner. NDP inhibited Hela cells more than Siha cells. FCM showed that the apoptosis rate of Hela cells and Siha cells increased with time and concentration, (P <0.05). The percentage of cells in S phase increased, the proportion of G0 / G1 phase decreased, while the proportion of G2 / M phase did not change obviously. Compared with control group Compared with Caspase 3, Caspase 9 expression increased, MMP-2 expression decreased. Conclusion: NDP can inhibit the proliferation of cervical adenocarcinoma cell Hela and squamous cell Si-ha, and NDP can inhibit Hela cell in vitro. The mechanism of NDP is related to the induction of apoptosis, cell cycle arrest, and the up-regulation of the expression of Caspase3 and Caspase9. Down-regulation of MMP-2 may be helpful to reduce invasiveness of cervical cancer cells.