目的 :探讨超氧化物歧化酶 (SOD)和白细胞介素 - 6 (IL - 6 )、白细胞介素 - 8(IL - 8)、肿瘤坏死因子 -α(TNF-α)在分泌性中耳炎发生和转归中的作用。方法 :检测 74例 (90耳 )分泌性中耳炎患者 (中耳炎组 )血浆和中耳积液中 SOD、IL- 6、IL- 8及 TNF- α,并以 30例健康人血浆作对照。结果 :中耳炎组 SOD、IL- 6、IL- 8及 TNF- α在中耳积液中阳性表达率分别为 88.9%、86 .8%、81.5 %和 74 .6 % ;其血浆含量均较对照组高 (P <0 .0 5 )。中耳炎组中耳积液的含量均较血浆中高 (P <0 .0 1)。病程短者 IL- 6和 IL- 8含量较病程长者高 (P <0 .0 1、 P <0 .0 5 ) ;病程长者 SOD及 TNF- α含量较病程短者高 (P <0 .0 1、 P <0 .0 5 )。浆液性积液者 IL- 6和 IL- 8含量较粘液性积液者高 (P <0 .0 1、 P <0 .0 5 ) ;粘液性积液者 SOD及 TNF-α含量较浆液性积液者高 (P <0 .0 1、 P <0 .0 5 )。 SOD含量与 TNF-α含量正相关 (r =0 .5 87,P <0 .0 1)。结论 :IL - 6和 IL - 8在分泌性中耳炎发病早期参与机体的防御反应 ,促进浆液性中耳积液产生 ;SOD和 TNF-α与疾病的持续状态相关 ,并参与中耳积液中粘蛋白的分泌 Objective: To investigate the effects of superoxide dismutase (SOD) and interleukin - 6 (IL - 6), interleukin - 8 (IL - 8) and tumor necrosis factor - The role of the outcome. Methods: The levels of SOD, IL-6, IL-8 and TNF-α in plasma and middle ear effusion of 74 patients with otitis media with otitis media (otitis media group) were measured. Thirty healthy controls were used as controls. Results: The positive expression rates of SOD, IL-6, IL-8 and TNF-α in middle ear effusion were 88.9%, 86.8%, 81.5% and 74.6% in the otitis media group, respectively. Group high (P <0. 05). The contents of middle ear effusion in otitis media group were higher than those in plasma (P <0.01). The levels of IL-6 and IL-8 in the short course were higher than those in the longer one (P <0.01, P <0.05) .0 1, P <0. 0 5). Serum IL-6 and IL-8 levels were higher than those in the mucus (P <0.01, P <0.05), while the levels of SOD and TNF-α in the serous fluid were higher than those of the serous Effusions were higher (P <0.01, P <0.05). The content of SOD was positively correlated with the content of TNF-α (r = 0.587, P <0.01). CONCLUSION: IL - 6 and IL - 8 are involved in the defensive reaction of the body in the early stage of secretory otitis media and promote the production of serous effusion. The activities of SOD and TNF - α are correlated with the persistent state of the disease, Protein secretion