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作者测定了一系列大肠杆菌克隆的流感病毒基因产物增强人类自然细胞毒性的能力。将不能粘附在尼龙毛柱上的人外周血单个核细胞(PBMC)置37℃培养过夜,培养物中加入大肠杆菌克隆产生的流感病毒基因产物,所用的靶细胞为骨髓白血病细胞系K562,粘附人类黑素瘤细胞系A375,两个结肠直肠癌细胞系(SW742和COLO205)以及伯基特淋巴瘤细胞系。通过~(51)Cr释放试验观察流感病毒基因产物对人类自然杀伤细胞毒性的增强作用。实验证明,不能粘附于尼龙毛柱的PBMC对多个克隆的病毒蛋白刺激均有应答反应。
The authors determined the ability of a range of E. coli cloned influenza virus gene products to enhance natural cytotoxicity in humans. Human peripheral blood mononuclear cells (PBMCs), which can not adhere to a nylon fleece column, were cultured overnight at 37 ° C. The resulting influenza virus gene product from the colony of E. coli was used in the culture. The target cells used were myeloid leukemia cell line K562, Adherence to human melanoma cell line A375, two colorectal cancer cell lines (SW742 and COLO205), and Burkitt’s lymphoma cell line. The enhancement effect of influenza virus gene products on human natural killer cytotoxicity was observed by ~ (51) Cr release test. Experiments have shown that PBMCs that can not adhere to nylon hair columns respond to viral protein stimulation of multiple clones.