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目的研究不同前处理方法对鲜葡萄Vitis vinifera枝蔓茋类化合物的影响。方法采用HPLC法同时测定葡萄枝蔓中活性成分白藜芦醇和ε-葡萄素,对新剪枝的葡萄枝蔓进行不同方式的干燥处理:阴干、烘干、冷藏;对葡萄枝蔓采取整枝、切片和粉碎3种方式处理,考察干燥过程中葡萄枝蔓中白藜芦醇和ε-葡萄素的动态变化规律。结果新鲜葡萄枝蔓中白藜芦醇和ε-葡萄素的量均较低,采用整枝阴干的前处理方式,白藜芦醇随着阴干时间的延长而发生显著变化,由初始的0.063 mg/g上升至7.175 mg/g,ε-葡萄素也由初始的0.838 mg/g上升至4.225 mg/g;二者均随时间延长达到一定程度后又呈现下降的趋势。而对于采用将葡萄枝蔓进行切片阴干、切片冷藏、粉碎阴干、粉碎冷藏和不同温度快速烘干处理,白藜芦醇和ε-葡萄素变化不明显。结论不同的处理方式能影响到剪枝后葡萄枝蔓中的白藜芦醇和ε-葡萄素量的变化。采取整枝阴干处理,白藜芦醇和ε-葡萄素随时间的延长均呈现先显著升高再下降的变化趋势,白藜芦醇和ε-葡萄素在一定阶段能达到可开发利用的水平。
Aim To study the effects of different pretreatment methods on the Vitis vinifera var. Methods Simultaneous determination of resveratrol and ε-glucuronidase in grape vine by HPLC method was carried out to dry the newly pruning grape vine: dried in the trunk, dried and refrigerated; pruning, slicing and grinding 3 ways to investigate the drying process of grapes vine Man Resveratrol and ε-grapevine dynamic changes. Results The amount of resveratrol and ε-glucuronide in fresh vine was lower than that in the control. Resveratrol was significantly changed with the prolongation of drying time by the pruning of the trunk. From the initial 0.063 mg / g To 7.175 mg / g and ε-grape increased from 0.838 mg / g to 4.225 mg / g, respectively. Both of them showed a trend of declining after reaching a certain extent with time. However, there was no obvious change of resveratrol and ε-grapevine in the sections of grape vine, dried in slices, frozen in slices, dried in shatter, crushed and refrigerated and quickly dried at different temperatures. Conclusion Different treatments can affect the amount of resveratrol and ε-grape in the branches and grapes of pruning. With the function of pruning, the resveratrol and ε-glucuronidase showed a trend of first increasing and then decreasing. The resveratrol and ε-grapein could reach the level of development and utilization at a certain stage.