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目的:研究清开灵对阿尔茨海默病(AD)模型小鼠脑组织β-淀粉样蛋白(Aβ)水平的影响。方法:选用雄性KM小鼠60只,随机分成6组:正常对照组、模型对照组、清开灵低、中、高剂量(20,40,80 g·kg-1)组和阳性对照药尼莫地平0.3 g·kg-1组,每组10只。ig给予等体积药物或生理盐水,每日给药1次,连续给药30 d。末次给药30 min后,东莨菪碱造模,用Morris水迷宫测试行为学指标,采用双抗体夹心法测定脑组织β-淀粉样蛋白水平。结果:Morris水迷宫测试表明,清开灵能明显缩短模型小鼠游泳时间及游泳路程;脑组织Aβ含量测定显示清开灵能显著降低脑组织Aβ水平。结论:清开灵能改善造模小鼠的学习记忆功能,其机制可能与降低脑组织Aβ水平有关。
Objective: To investigate the effect of Qingkailing on the level of β-amyloid protein (Aβ) in brain of Alzheimer disease (AD) model mice. Methods: Sixty male KM mice were randomly divided into 6 groups: normal control group, model control group, Qingkailing low, medium and high dose groups (20,40,80 g · kg -1) and positive control group Mopidipine 0.3 g · kg-1 group, 10 rats in each group. ig given an equal volume of drugs or saline, administered once daily for 30 d. Scopolamine was given to rats for 30 min after the last administration, Morris water maze test was used to test behavioral indexes, and β-amyloid level in brain tissue was measured by double antibody sandwich method. Results: Morris water maze test showed that Qingkailing can obviously shorten the swimming time and swimming distance of the model mice. The Aβ content in the brain tissue showed that Qingkailing can significantly reduce the level of Aβ in the brain tissue. Conclusion: Qingkailing can improve the learning and memory function of model mice, the mechanism may be related to the decrease of Aβ level in brain tissue.