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为研究60Coγ-射线辐照预处理对酶解魔芋葡甘聚糖片段体内、外抗肿瘤活性的影响,将魔芋精粉经60Coγ-射线辐照后加β-甘露聚糖酶酶解(KF2),与未辐照预处理的酶解物(KF1)通过体内、外抑瘤实验,研究KF1、KF2对Hela细胞体外生长的抑制作用及对EAC腹水癌小鼠的体内抑瘤率、存活期、脾脏指数、胸腺指数、腹腔巨噬细胞活性的影响.结果表明:γ-射线辐照预处理使KGM酶解更彻底;随着KF1、KF2浓度及培养时间的增加,He-la细胞存活率逐渐降低,且KF2作用高于KF1;KF1、KF2对EAC腹水癌小鼠脏器指数及腹腔吞噬细胞活性均有不同程度的增强作用,移植性实体瘤抑瘤率增大、且60Coγ射线辐照预处理过的KF2各项指标均高于KF1,表明60Coγ射线辐照预处理增强了KGM酶解产物的抗肿瘤活性.
In order to study the effect of 60Coγ-ray irradiation pretreatment on the antitumor activity of konjac glucomannan in vitro and in vivo, the konjac flour was radiated with 60Coγ-ray and enzymatically hydrolyzed by β-mannanase (KF2) , And non-irradiated pretreatment enzyme (KF1) in vitro and in vivo anti-tumor experiments KF1, KF2 on Hela cells in vitro growth inhibition and EAC ascites tumor in mice in vivo inhibition rate, survival, Spleen index, thymus index and peritoneal macrophage activity.The results showed that γ-ray irradiation preconditioning made KGM more completely. With the increase of KF1, KF2 concentration and culture time, the survival rate of He-la cells gradually increased KF1 and KF2 enhanced the organ index and the activity of peritoneal phagocytes in mice with EAC ascites carcinoma. The tumor growth rate of transplant solid tumors increased, and the radiation of 60Co γ-ray KF2 treated indicators were higher than KF1, indicating that pretreatment of 60Co γ-ray enhanced KGM enzymatic activity of anti-tumor products.