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[目的]探讨HLA-DR9基因与新疆哈萨克族食管癌的关系,以及年龄在这种关系中的作用。[方法]运用序列特异性引物聚合酶链反应(PCR-SSP)检测120例哈萨克食管癌病例和240例对照的HLA-DR9等位基因频率,采用Mantel-Haenszel分层卡方检验、OR一致性检验及OR趋势检验进行统计分析。[结果]在哈萨克族食管癌组中,等位基因HLA-DR9的携带率为22.5%(27/120),在对照中,此等位基因的携带率为11.7%(28/240),两组之间的差异有统计学意义(χ2=7.25,P=0.007),OR值为2.20(95%CI:1.23～3.93)。按年龄分层经Mantel-Haenszel卡方检验,χ2M-H=7.16,P﹤0.05,调整后合并ORM-H为2.18(95%CI:1.23～3.85);﹤60岁年龄组HLA-DR9基因阳性与哈萨克族食管癌的OR值为1.17(95%CI:0.48～2.81);60～69岁年龄组OR值为3.37(95%CI:1.23～9.22);≥70岁年龄组OR值为4.93(95%CI:1.21～20.16),OR趋势卡方检验,χ2=4.04,P﹤0.05,各层OR有线性趋势。混杂与交互作用分析提示:调整后ORM-H与cOR值基本一致,层间OR齐性检验,χ2H=6.02,P﹤0.05,各层OR值不具同质性。[结论]HLA-DR9等位基因是新疆哈萨克族食管癌的易感基因,随年龄增加HLA-DR9阳性携带对哈萨克族食管癌的风险逐步增加,年龄在HLA-DR9阳性携带与哈萨克族食管癌关系中的混杂作用不明显,主要为效应修饰作用。
[Objective] To investigate the relationship between HLA-DR9 gene and Xinjiang Kazakh esophageal cancer and the role of age in this relationship. [Method] The frequency of HLA-DR9 alleles in 120 cases of Kazakh esophageal cancer and 240 controls was detected by PCR-SSP with Mantel-Haenszel stratified chi-square test. The OR consistency Test and OR trend test for statistical analysis. [Results] In Kazak esophageal cancer group, the allele HLA-DR9 carrying rate was 22.5% (27/120), in the control, the carrying rate of this allele was 11.7% (28/240), two The difference between the groups was statistically significant (χ2 = 7.25, P = 0.007), OR was 2.20 (95% CI: 1.23-3.93). According to age-stratified by Mantel-Haenszel Chi-square test, χ2M-H = 7.16, P <0.05, adjusted ORM-H was 2.18 (95% CI: 1.23 ~ 3.85); <60 years old group of HLA-DR9 gene positive OR of Kazak esophageal cancer was 1.17 (95% CI: 0.48-2.81); OR of 3.37 (95% CI: 1.23-9.22) in 60-69 years old group and 4.93 95% CI: 1.21-20.16). OR trend chi-square test was used, χ2 = 4.04, P <0.05. Hypothesis analysis showed that ORM-H was basically the same as cOR after adjustment, OR test was also used for OR between two groups, χ2H = 6.02, P <0.05. OR values of different layers were not homogeneity. [Conclusion] The allele of HLA-DR9 is a susceptible gene for Kazakh esophageal cancer in Xinjiang. With increasing age, the risk of carrying HLA-DR9 positive for Kazakh esophageal cancer gradually increases. The age of HLA-DR9 is positively associated with Kazakh esophageal cancer The mixed effect of the relationship is not obvious, mainly for the effect of modification.