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将含基因cry1C的质粒 ,通过电脉冲法转入含基因Cry1C的质粒 ,通过电脉冲法转入含基因Cry1Ab、Cry1Ac和cry2的对小菜蛾具有高毒力的苏云金芽胞杆菌野生菌株YBT 80 3 1中 ,得到转化子BMBY 0 0 3。PCR和SDS PAGE分析显示 ,Cry1C可在其中正常复制、表达 ,但使受体菌部分内源质粒发生丢失。生物测定结果表明 ,转化子BMBY 0 0 3既对甜菜夜蛾有毒力 ,LC50 值为 1 1 78μL/mL ,高于出发菌株YBT 80 3 1 (LC50 1 879μL/mL) ,也对小菜蛾有毒力 ,LC50 值为 1 96 8μL/mL ,低于YBT 80 3 1 ((LC50 1 1 43 μL/mL)。表明cry1C转入后 ,提高了野生菌株YBT 80 3 1对甜菜夜蛾的毒力 ,却降低了对小菜蛾的毒力。
The plasmid containing cry1C gene was transformed into plasmid Cry1C by electroporation and transferred into wild strain Bacillus thuringiensis YBT 80 3 1 with high toxicity to the diamondback moth with the genes Cry1Ab, Cry1Ac and cry2 by electroporation method The transformant BMBY 0 0 3 was obtained. PCR and SDS PAGE analysis showed that Cry1C could be replicated and expressed normally, but the endogenous plasmids of the recipient bacteria were lost. Bioassay results showed that the transformant BMBY 0 0 3 was both virulent to beet armyworm with LC50 value of 1 1 78 μL / mL, which was higher than that of the parent strain YBT 80 3 1 (LC50 1 879 μL / mL) and virulent to diamondback moth , The LC50 value was 1 96 8 μL / mL, lower than that of YBT 80 3 1 (LC50 1 1 43 μL / mL), indicating that the cry1C translocation increased the virulence of the wild strain YBT 80 3 1 against beet armyworm Reduce the toxicity to Plutella xylostella.