鉴于应用免疫放射试验(IRMA)检测蚊体内子孢子要有较复杂的条件设备,本文报导试用双部位酶联免疫吸附试验(ELISA)微量法进行检测子孢子的研究。抗原为子孢子阳性蚊,以大劣按蚊叮咬感染诺氏疟原虫(H株)的恒河猴或弗氏按蚊叮咬感染伯氏疟原虫(ANKA株)的仓鼠后14～16天将蚊杀死而得,置室温干燥器内保存8周后使用。抗血清为对诺氏疟原虫子孢子特异的单克隆抗体(2G3),先以DEAE-Se- In order to detect the sporozoites in mosquitoes by using the immune radiation assay (IRMA), we need to investigate the sporozoites in the mosquito by using the two-site enzyme-linked immunosorbent assay (ELISA). The antigen is a sporozoite-positive mosquito bites an rhesus macaque infected with Plasmodium knowlesii (strain H) or an anopheles stephen mosquito bites seizures of hamsters infected with P. berghei (ANKA strain) 14 to 16 days after mosquito breeding Kill it, set the room temperature dryer for 8 weeks before use. The antiserum is a monoclonal antibody specific for sporozoites of Plasmodium knowlesi (2G3), first with DEAE-Se-