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研究显示,流感病毒在体内可诱导人体的呼吸道上皮细胞凋亡,在体外使宫颈癌细胞(Hela)和狗肾传代细胞(MDCK)凋亡[1,2]。近来报道抗流感病毒药物可抑制流感病毒诱导感染细胞凋亡[3]。但双黄连是否可抑制流感病毒诱导MDCK细胞凋亡少有报道。我们自2005年7月至2008年6月采用Annexin V及碘化丙啶(PI)双染并应用流式细胞仪检测双黄连对流感病毒诱导MKCK细胞凋亡的影响,报告如下。
Studies have shown that influenza virus induces apoptosis of human respiratory epithelial cells in vivo and induces apoptosis of cervical cancer cells (Hela) and dog kidney-passaging cells (MDCK) in vitro [1, 2]. Recently, it has been reported that anti-influenza virus drugs can inhibit influenza virus-induced apoptosis in infected cells [3]. However, whether Shuanghuanglian can inhibit influenza virus-induced MDCK cell apoptosis is rarely reported. We used Annexin V and propidium iodide (PI) double staining from July 2005 to June 2008 to detect the effect of Shuanghuanglian on influenza virus-induced MKCK cell apoptosis by flow cytometry. The report is as follows.