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1963年Schmid用氚标记胸腺嘧啶放射自显影方法,观察细胞分裂中期染色体DNA复制情况时发现正常女性的两条X染色体中有一条复制较迟。此后在细胞遗传学领域中又推出多种简便的制备迟复制X染色体(Lx)的方法用于研究各种性发育异常患者的性染色体组成和迟复制X染色体的结构与功能。我室参考文献上的方法,采用BrdU参入—吖啶橙荧光染色—紫外线光解-Giemsa染色技术,对1985年从中期妊娠引产孕妇得到的30份羊水标本进行了观察。材料与方法一、标本:男胎羊水标本13例,女胎17例。二、试剂: 1.0.005％BrdU液:称取:BrdU(Fluka)2mg,加去离子水4ml,再用去离子水稀
In 1963, Schmid used tritium labeled thymidine autoradiography to observe the replication of metaphase chromosomal DNA in late metaphase. One of the two X chromosomes in normal women was found to be late in replication. Since then in the field of cytogenetics has introduced a variety of simple preparation of late replication X chromosome (Lx) method for the study of sexual dysplasia in patients with sex chromosome composition and late replication X chromosome structure and function. My room reference method, the use of BrdU incorporation - acridine orange fluorescence staining - UV photolysis - Giemsa staining, induction of labor in mid-pregnancy in 1985 were 30 cases of amniotic fluid specimens were observed. Materials and methods First, specimens: male fetus amniotic fluid specimens in 13 cases, female fetuses in 17 cases. Second, reagents: 1.0.005% BrdU solution: Weigh: BrdU (Fluka) 2mg, add deionized water 4ml, then diluted with deionized water