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采用苗期群体鉴定法对供试水稻品种进行了抗性鉴定,在此基础上测定了取食不同抗性水稻品种的褐稻虱(Nilaparvata lugens)羧酸醑酶、酸性及碱性磷酸酯酶活力的变化。结果表明,取食感虫品种的褐稻虱若虫及第2代若虫体内上述三种酶的活力明显高于取食抗虫品种的;取食中抗品种的褐稻虱若虫三种酶的活力介于取食感虫和抗虫品种的若虫之间。这一结果与褐稻虱在不同抗性水平上水稻品种的存活率和发育进度是一致的。表明抗虫品种对褐稻虱具有抗生机制,从而抑制了虫体的正常生长发育。取食不同水稻品种的褐稻虱的羧酸酯酶米氏常数(Km)值无明显差异,但最大反应速度(Vmax)值差异极显著。取食感虫品种的 Vmax 值明显高于取食抗虫品种的。这表明取食不同抗性水稻品种的褐稻虱羧酸酯酶性质相同,差异主要是由于酶量的不同。
The seedling population identification method was used to test the resistance of the tested rice varieties. On the basis of this, the activities of carboxylase, acidity and alkaline phosphatase of Nilaparvata lugens fed on different resistant rice varieties were determined. Vibrant changes. The results showed that the activities of the above three enzymes in the nymphs and the second generation nymphs of the feeding insects were significantly higher than that of the feeding insect-resistant ones. The activities of three enzymes of the brown planthopper nymphs Between feeding insects and insects varieties between the nymphs. This result is consistent with the survival rate and developmental progress of rice cultivars at different resistance levels. The results showed that the insect-resistant varieties had an anti-microbial mechanism on the brown planthopper, which inhibited the normal growth and development of the insect body. There was no significant difference in carboxylesterase (Km) values of the brown planthopper from different rice varieties, but the maximum response rate (Vmax) was significantly different. Vmax values of feeding susceptible varieties were significantly higher than those of feeding insect-resistant varieties. This indicated that the properties of the carboxylesterase of the brown planthopper in feeding different resistant rice varieties were the same, the difference mainly due to the difference of enzyme amount.