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三维激光扫描技术建立的植物三维立体模型,可精确、全面、原位地反映植物形态特征,对于揭示接菌植物的微观形态特性具有比传统测量方法更明显的优势。以玉米为供试材料,在低磷条件下(20 mg/kg),设置接种菌根与对照两个处理,研究三维激光扫描技术对接种丛枝菌根真菌的植株形态特征在不同生长期的动态变化规律,分析比较不同处理的植株侵染率、菌丝密度、土壤酸性磷酸酶活性和土壤有效磷等理化指标及玉米形态特征的变化。研究结果表明,在低磷条件下丛枝菌根在30 d时侵染率已经达到82%,菌丝密度增加,酸性磷酸酶活性增强。在生长到30 d的时候,接菌组的株高、冠幅分别比对照组提高52%,47%,生长到60 d时接菌组茎杆横截面积为对照组的124%,玉米活叶面积、叶宽、叶片垂高等都得到提高,并且随着生长期延长接菌和对照处理的差异增大。以曲率半径表示叶片弯曲程度,在生长到60 d时接菌组曲率半径比对照组增加46.5%,接种丛枝菌根真菌促使叶片更加坚挺。
The three-dimensional model of plant established by three-dimensional laser scanning technology can accurately, completely and in situ reflect the morphological characteristics of plants, revealing the microscopic morphological characteristics of the inoculated plants has more obvious advantages than the traditional measurement methods. Maize was used as test material to inoculate mycorrhizal and control under low phosphorus (20 mg / kg) treatment. The effects of three-dimensional laser scanning on the morphological characteristics of the plants inoculated with arbuscular mycorrhizal fungi at different growth stages Dynamic changes, the changes of plant infection rate, mycelium density, soil acid phosphatase activity and soil available phosphorus and other physical and chemical indicators and morphological characteristics of maize were analyzed and compared. The results showed that under low phosphorus conditions, the infection rate of arbuscular mycorrhizae reached 82% at 30 d, the mycelial density increased and the activity of acid phosphatase increased. After 30 days of growth, the plant height and crown width of the inoculation group were increased by 52% and 47% respectively compared with the control group, and the cross-sectional area of the stem of the inoculation group was 124% of the control group when grown to 60 days. Leaf area, leaf width, leaf vertical height and so on have been improved, and with the extension of the growing period of inoculation and control treatment increased. The curvature radius indicates the degree of leaf bending. After 60 days of growth, the radius of curvature of the inoculated group increased 46.5% than that of the control group. Inoculation of arbuscular mycorrhizal fungi promoted the leaf to be more firm.