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目的采用HPLC法测定苏菲咳糖浆中的盐酸麻黄碱。方法色谱柱为Agilent C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.02 mol.L-1磷酸二氢钾溶液(含0.1%三乙胺,磷酸调pH2.7)(4∶100);检测波长210 nm,流速1.0 mL.min-1,柱温35℃。结果盐酸麻黄碱进样量0.12～1.20μg与峰面积值呈良好的线性关系(r=0.9999),平均回收率为99.1%,RSD=1.74%(n=6)。结论所用方法操作简便、准确、灵敏度高、重复性好,能有效地检测苏菲咳糖浆中的盐酸麻黄碱。
Objective To determine ephedrine hydrochloride in Sufever cough syrup by HPLC. Methods The column was Agilent C18 column (250 mm × 4.6 mm, 5 μm) and the mobile phase was acetonitrile-0.02 mol·L-1 potassium dihydrogen phosphate solution containing 0.1% triethylamine and phosphoric acid at pH 2.7 100). The detection wavelength was 210 nm and the flow rate was 1.0 mL.min-1. The column temperature was 35 ℃. Results The linear range was 0.12 ~ 1.20μg for ephedrine hydrochloride (r = 0.9999). The average recovery was 99.1% with RSD = 1.74% (n = 6). Conclusion The method used is simple, accurate, sensitive, reproducible and effective for the determination of ephedrine hydrochloride in Sufever cough syrup.