[Objective]To study the system of aseptic seeding and rapid propogationtin of hybrid seeds of Demdrobium officinale and Dendrobium aduncum [Methods] Demdrobium officinale and Dendrobium aduncum were the objects of research,and their hybrid seeds were gained by artificially auxiliary hybridation. The seeds were induced to germinate in vitro,and study on the technology of aseptic seeding was finished. [Results]Successful hybridation of Demdrobium officinale and Dendrobium aduncum could be easily realized,and hybrid fruit-bearing rate could reach 80%; the suitable culture medium for seeds was: MS +1. 0 mg/L 6-BA +0. 1 mg/L NAA,the germination rate after 30 d of cultivation was 89. 7%; appropriate culture medium for proliferation of protocorm was: MS + 1. 5 mg /L 6-BA + 0. 1 mg /L NAA + 100 g /L banana + 1. 0 mg /L AC; appropriate culture medium for differentiation of protocorm was: MS + 1. 5 mg /L 6-BA + 0. 1 mg /L NAA + 200 g /L potato + 1. 0 g /L AC; the formula of culture medium for strenghening the seedlinge and nurturing the rooting was: 1 /2 MS + 0. 6 mg /L NAA +100 g /L banana + 1. 0 g /L AC,the plant grew robustly,the rooting percentage was 100%,and the survival rate of transplanting was higher than 95%. [Conclusions]This method ascertains the optimum culture media formula in the whole regeneration process and all phases from seed germination to acclimatization and transplantation,so fine solidation is laid for its germplasm innovation and industrialized seedling production. [Objective] To study the system of aseptic seeding and rapid propogationtin of hybrid seeds of Demdrobium officinale and Dendrobium aduncum [Methods] Demdrobium officinale and Dendrobium aduncum were the objects of research, and their hybrid seeds were gained by artificially auxiliary hybridization. The seeds were induced to germinate in vitro, and study on the technology of aseptic seeding was finished. [Results] Successful hybridization of Demdrobium officinale and Dendrobium aduncum could be easily realized, and hybrid fruit-bearing rate could reach 80%; the suitable culture medium for seeds was: MS +1. 0 mg / L 6-BA +0.1 mg / L NAA, the germination rate after 30 d of cultivation was 89.7%; appropriate culture medium for proliferation of protocorm was: MS + 1.5 mg / L 6-BA + 0. 1 mg / L NAA + 100 g / L banana + 1.0 mg / L AC; appropriate culture medium for differentiation of protocorm was: MS + 1.5 mg / L 6-BA + 0. 1 mg / L NAA + 200 g / L potato + 1.0 g / L AC; the formula of culture medium for st renghening the seedlinge and nurturing the rooting was: 1/2 MS + 0.6 mg / L NAA + 100 g / L banana + 1.0 g / L AC, the plant grew robustly, the rooting percentage was 100%, and the survival rate of transplanting was higher than 95%. [Conclusions] This method ascertains the optimum culture media formula in the whole regeneration process and all phases from seed germination to acclimatization and transplantation, so fine solidation is laid for its germplasm innovation and industrialized seedling production .