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建立了硅烷化衍生化法结合气相色谱/质谱(GC/MS)法同时测定卷烟中1,2-丙二醇、甘油和糖类物质含量的分析方法。样品以N,N-二甲基甲酰胺(DMF)和N,O-双(三甲基硅烷基)三氟乙酰胺(BSTFA)为提取溶剂和衍生化试剂,于70℃衍生化反应30 min,采用DB-5MS色谱柱(60 m×0.25 mm×0.25μm),用GC/MS分析测定1,2-丙二醇,甘油、果糖、葡萄糖和蔗糖的含量。方法线性关系良好,检出限为0.04~2.85 mg/g,定量限为0.13~9.51 mg/g,平均加标回收率为90.3%~114.4%,相对标准偏差小于9.1%。方法适合于卷烟样品中1,2-丙二醇、甘油和糖类物质的同时测定。
A method for the simultaneous determination of 1,2-propanediol, glycerol and carbohydrate in cigarettes by silylation derivatization coupled with gas chromatography / mass spectrometry (GC / MS) was established. The sample was derivatized with N, N-dimethylformamide (DMF) and N, O-bis (trimethylsilyl) trifluoroacetamide (BSTFA) as extraction solvent and derivatization reagent at 70 ℃ for 30 min The contents of 1,2-propanediol, glycerol, fructose, glucose and sucrose were determined by GC / MS using a DB-5MS column (60 m × 0.25 mm × 0.25 μm) The method has a good linearity. The detection limit is 0.04-2.85 mg / g, the limit of quantification is 0.13-9.51 mg / g, the average recovery is 90.3% -114.4% and the relative standard deviation is less than 9.1%. The method is suitable for the simultaneous determination of 1,2-propanediol, glycerol and carbohydrates in cigarette samples.