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目的:探讨BAG-1、CDK4蛋白在结直肠癌中的表达及其与结直肠癌临床病理特征的关系。方法:应用免疫组化SP法对80例结直肠癌组织、40例结直肠腺瘤组织及25例癌旁正常结直肠组织中的BAG-1、CDK4进行检测。结果:BAG-1、CDK4在结直肠癌中过度表达,阳性率分别为80.0%(64/80)和65.0%(52/80),并且BAG-1、CDK4与肿瘤的分化程度成反比。有淋巴结转移的结直肠癌BAG-1、CDK4的阳性率分别为96.7%(29/30)和83.3%(25/30),无淋巴结转移的结直肠癌阳性率分别为70.0%(35/50)和54.0%(27/50),两者比较,差异有高度统计学意义(P<0.01)。侵及浆膜层者CDK4蛋白表达阳性率明显高于未侵及浆膜层者,阳性率分别为71.4%(40/56)和35.3%(12/34),差异有高度统计学意义(P<0.01)。结论:BAG-1、CDK4的过度表达与肿瘤的分化程度、淋巴结转移密切相关;CDK4的过度表达还与浸润深度密切相关;结直肠癌的发生机制涉及BAG-1、CDK4调节环路中多个基因的异常。
Objective: To investigate the expression of BAG-1 and CDK4 protein in colorectal cancer and its relationship with clinicopathological features of colorectal cancer. METHODS: Immunohistochemical SP method was used to detect BAG-1 and CDK4 in 80 cases of colorectal cancer, 40 cases of colorectal adenoma and 25 cases of normal colorectal adenoma. RESULTS: BAG-1 and CDK4 were over-expressed in colorectal cancer, the positive rates were 80.0% (64/80) and 65.0% (52/80) respectively, and BAG-1 and CDK4 were inversely proportional to tumor differentiation. The positive rates of BAG-1 and CDK4 in colorectal cancer with lymph node metastasis were 96.7% (29/30) and 83.3% (25/30), respectively. The positive rate of colorectal cancer without lymph node metastasis was 70.0% (35/50). Compared with 54.0% (27/50), the difference was statistically significant (P<0.01). The positive rate of CDK4 protein expression in the invaded serosal layer was significantly higher than that in the non-invaded serosal layer. The positive rates were 71.4% (40/56) and 35.3% (12/34) respectively, and the difference was highly statistically significant (P <0.01). Conclusion: The overexpression of BAG-1 and CDK4 is closely related to the degree of tumor differentiation and lymph node metastasis. The overexpression of CDK4 is also closely related to the depth of invasion. The mechanism of colorectal cancer involves more than one in the BAG-1 and CDK4 regulatory loops. Gene abnormalities.