本文提出一种米粒不经磨粉、煮沸,直接用冷碱液在30℃下糊化溶解后测定其直链淀粉含量的单粒稻米直链淀粉含量测定法。本法测得的结果与常规方法基本一致,并具有简便易行,工作量小,样品用量少等优点,可应用于稻米直链淀粉的生理和遗传方面的研究,为优质育种的后代筛选提供有效手段。 In this paper, a single grain rice amylose content determination method was proposed to determine the amylose content of rice grains without milling and boiling, and directly after gelatinization and dissolving with cold caustic soda at 30 ℃. The results obtained by this method are basically the same as conventional methods and have the advantages of simple and easy operation, small workload, less sample amount, etc., which can be applied to the physiological and genetic studies of amylose in rice so as to screen the offspring of high quality breeding Provide effective means.