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为了探讨抗氧化剂对造血干细胞在低温条件下损伤的防护作用,将小鼠骨髓细胞置于4℃条件下保存,观察在保养液中加入不同浓度的Cu、Zn、SOD对细胞死伤的防护效果和马血清对细胞回收率的影响。结果表明,含20%的马血清保养液中加入SOD1.65U或0.165U/ml,保存3天,CFU-GM、CFU-E、BFU-E、CFU-Meg、CFU-Mix的产率分别为对照组(不加SOD)的6.2、2.6、2.9、4.0和5.1倍,明显提高了造血祖细胞的活存率,其中CFU-GM和CFU-Mix达到保存前水平。SOD的有效作用机理不是对造血祖细胞增殖调控作用,而是防护了细胞的死亡。这可能与其清除过氧自由基的抗氧化作用有密切关系。
In order to investigate the protective effect of antioxidants on the injury of hematopoietic stem cells under the condition of low temperature, the mouse bone marrow cells were stored at 4 ℃, and the protective effect of Cu, Zn and SOD with different concentrations on the cell death and injury were observed. Effect of Horse Serum on Cell Recovery. The results showed that the yields of CFU-GM, CFU-E, BFU-E, CFU-Meg and CFU-Mix after 20% of the horse serum maintenance solution were added with SOD1.65U or 0.165U / Which was 6.2, 2.6, 2.9, 4.0 and 5.1 times higher than control group (without SOD), significantly increased the survival rate of hematopoietic progenitor cells, in which CFU-GM and CFU-Mix reached Pre-save level. The effective mechanism of action of SOD is not to regulate the proliferation of hematopoietic progenitor cells, but to protect the cells from death. This may be related to its elimination of peroxidation of free radicals are closely related to the role of antioxidants.