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目的探讨血清可溶性CD 3 0(s CD 3 0)作为电离辐射剂量估算的生物指标的可行性。方法健康雄性C57BL/6小鼠300只,随机分为对照组及1、3、5、7Gy辐照组(n=60)。辐照组小鼠接受60Coγ射线一次性全身辐照,剂量率为0.78Gy/min。各组分别于辐照后24、48、72h留取血清样本(每组每时间点20只小鼠),采用定量抗体芯片检测s CD30浓度,并通过回归分析法分析血清s CD30浓度与辐照剂量的相关性。结果未辐照小鼠s CD30正常值范围为121.2±25.6pg/ml;辐照后24、48、72h,血清s CD30浓度随辐射剂量增大而降低。回归分析显示,血清s CD30浓度与辐照剂量具有明显的剂量-效应关系(R~2=0.9913),其回归方程为y=1.1489x~2-21.139x+121.44。结论小鼠血清s CD30表达基线稳定,其浓度变化与急性辐照剂量相关,可作为γ电离辐射剂量估算的生物指标。
Objective To investigate the feasibility of serum soluble CD 3 0 (s CD 3 0) as a biomarker for ionizing radiation dose estimation. Methods 300 healthy male C57BL / 6 mice were randomly divided into control group and 1,3,5,7Gy irradiation group (n = 60). The irradiated mice received a one-time whole-body irradiation with 60Co γ-rays at a dose rate of 0.78 Gy / min. Serum samples (20 mice at each time point) were collected at 24, 48, and 72 hours after irradiation respectively. The CD30 levels of sCD30 were detected by quantitative antibody chip, and the concentrations of serum s CD30 and irradiation were analyzed by regression analysis Correlation of dosages. Results The normal range of CD30 in non-irradiated mice was 121.2 ± 25.6pg / ml. At 24, 48 and 72h after irradiation, the serum CD30 levels decreased with the increase of radiation dose. Regression analysis showed that there was a significant dose-effect relationship between serum CD30 concentration and irradiation dose (R ~ 2 = 0.9913). The regression equation was y = 1.1489x ~ 2-21.139x + 121.44. Conclusions The expression of CD30 in serum of mice is stable at baseline. The change of concentration of CD30 in serum is correlated with the dose of acute radiation, which can be used as a biomarker to estimate dose of γ ionizing radiation.