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由于新发现的流感病毒抗体CR6261可以中和多种亚型的流感病毒,而且是其VH区结合于流感病毒蛋白HA的保守区,因此该抗体有望成为一种广谱的流感治疗性抗体而备受关注。通过构建真核表达载体pCR6261VH、pCR6261VH-GFP、pCR6261scFv,成功地筛选到在细胞膜上稳定表达CR6261VH、CR6261VH-GFP与CR6261scFv的单克隆细胞系。用流感病毒去感染稳定细胞系,间隔12 h取上清检测病毒血凝效价。结果表明:稳定表达CR6261scFv和CR6261VH-GFP细胞系能够降低病毒的血凝效价,而表达CR6261VH细胞系的血凝效价与对照无显著差异。说明CR6261VH与CR6261scFv在细胞膜上表达能够部分抑制病毒侵染细胞。尽管更有效的抑制策略还在研究中,但本研究将为抗流感转基因动物的育种提供新的思路。
Because the newly discovered influenza virus antibody CR6261 neutralizes influenza viruses of many subtypes and binds its VH region to the conserved region of the influenza virus protein HA, the antibody is expected to become a broad-spectrum therapeutic antibody against influenza Concerned. The monoclonal cell lines stably expressing CR6261VH, CR6261VH-GFP and CR6261scFv were successfully screened by constructing eukaryotic expression vectors pCR6261VH, pCR6261VH-GFP and pCR6261scFv. The influenza virus was used to infect the stable cell line, and the supernatant was taken at 12 h intervals to test the virus titer. The results showed that stable expression of CR6261scFv and CR6261VH-GFP cell line can reduce the titer of the virus, while the expression of CR6261VH cell line titer was no significant difference with the control. This indicates that the expression of CR6261VH and CR6261scFv on the cell membrane can partially inhibit the virus infecting cells. Although more effective suppression strategies are still under study, this study will provide new ideas for the breeding of anti-influenza transgenic animals.