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目的探讨C57BL/6小鼠严重烫伤延迟复苏后肺组织中白介素21(IL-21)的变化及其意义。方法雄性C57BL/6小鼠100只,随机分为延迟复苏组(DFR组,n=40)、即时复苏组(IFR组,n=50)和正常对照组(CG组,n=10),建立体表面积25%的Ⅲ度烫伤模型,分别于致伤后1、6、24、72 h和168 h取材。采用组织病理学、免疫组织化学染色、图像分析技术,观察不同实验组小鼠肺脏组织的病理学变化与IL-21的表达。结果 IL-21的阳性表达位于肺泡上皮细胞的胞核与胞质,延迟复苏组与即时复苏组IL-21的表达分别明显高于正常对照组,且延迟复苏组小鼠肺组织IL-21的表达高于即时复苏组小鼠肺组织IL-21的表达(P<0.05)。结论 IL-21表达强度的变化与严重烫伤延迟复苏小鼠肺组织损伤有关。
Objective To investigate the changes of interleukin-21 (IL-21) in lung tissue of C57BL / 6 mice after delayed resuscitation with severe scald and its significance. Methods One hundred male C57BL / 6 mice were randomly divided into delayed resuscitation group (DFR group, n = 40), immediate resuscitation group (IFR group, n = 50) and normal control group (n = 10) The third degree scald model with body surface area of 25% were drawn at 1, 6, 24, 72 h and 168 h after injury respectively. Histopathology, immunohistochemical staining and image analysis were used to observe the pathological changes and IL-21 expression in lung tissue of different experimental groups. Results The positive expression of IL-21 was located in the nucleus and cytoplasm of alveolar epithelial cells. The expression of IL-21 in delayed resuscitation group and immediate resuscitation group were significantly higher than that in normal control group, and IL-21 in lung tissue of delayed resuscitation group The expression of IL-21 in the lung tissue of mice in immediate recovery group was significantly higher than that in normal mice (P <0.05). Conclusion The change of IL-21 expression is related to the lung injury in mice with delayed resuscitation.