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给大鼠腹腔注射大肠杆菌内毒素(endotoxin, ET)复制发热模型,观察大鼠ET性发热时不同脑区组织cAMP含量和腺苷酸环化酶(adenylate cyclase, AC)活性的变化。结果发现:大鼠在发热高峰时与对照组比较,丘脑下部cAMP含量明显增加(P<0.01),并与体温变化呈正相关关系(r=0.827);丘脑下部AC活性也显著增强(P<0.001),也与体温变化呈正相关关系(r=0.774)。脑干AC活性显著增强(P<0.05),但与体温变化无正相关关系(r=0.203),cAMP含量也无明显变化。脑皮质cAMP含量和AC活性均无明显变化。以上结果显示:ET可能是通过共同信息物质——内生致热原(EP),以一定方式作用于丘脑下部视前区神经元的细胞膜内AC,使其活化作用于ATP,使ATP分解生成cAMP,从而使局部cAMP含量增加,再通过某种方式使体温调定点上移,导致体温升高。
Rats were injected intraperitoneally with endotoxin (ET) model to observe the changes of cAMP content and adenylate cyclase (AC) activity in different brain regions of ET rats. The results showed that the content of cAMP in the hypothalamus was significantly increased (P <0.01), and was positively correlated with body temperature (r = 0.827). The activity of AC in the hypothalamus was also significantly increased (P <0.001) ), But also a positive correlation with body temperature (r = 0.774). AC activity of brainstem increased significantly (P <0.05), but there was no positive correlation with body temperature (r = 0.203), and cAMP content did not change significantly. There was no significant change in cAMP content and AC activity in cerebral cortex. The above results show that ET may act on the AC in the plasma membrane of the preoptic neurons in the hypothalamus through a common information material, endogenous pyrogen (EP), activating it to activate ATP to generate ATP cAMP, so that the increase in local cAMP content, and then somehow the body temperature set point up, resulting in increased body temperature.