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众所周知在解剖蚊媒时,不仅可以从其头部和胸部收集到班氏丝虫、马来丝虫和彭亨丝虫的Ⅲ期幼虫(L3),而且可以从其腹部收集到上述L3。从流行病学考虑,测定从蚊媒腹部收集到的L3对终宿主是否有感染性是有意义的。作者用彭亨丝虫微丝蚴阳性的猫喂饲几百只2～6日龄雌性埃及伊蚊,在27℃～30℃,75%～90%湿度下饲养。感染后第7d乙醚麻醉解剖,分离埃及伊蚊腹部,置于含生理盐水的平皿中,收集L3。以每鼠130条L3经腹股沟皮下感染5只雄性长爪沙鼠。于感染后58,67,77d从眶窦采血、涂片、
It is well known that when disseminated mosquito vectors, not only Phase III larvae (L3) of Banday filarios, Malay filarias and Paenibacillus filarias can be collected from their heads and their breasts, but also the L3 described above can be collected from their belly. From an epidemiological point of view, it is of interest to determine whether L3 collected from the mosquito-borne belly is infectious to the final host. The authors fed several hundred cats of 2 to 6 day-old female Aedes aegypti with a penicillium-rich microflora of Paenace, and fed at 27 to 30 ° C and 75 to 90% humidity. After 7 days of infection, the rats were anesthetized and anesthetized. The belly of Aedes aegypti was isolated and placed in a plate containing saline, and L3 was collected. Five male gerbils were infected subcutaneously with 130 L3 per groin. 58,67,77 days after infection from the orbital sinus blood, smear,