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目前关于儿童原发性血小板增多症(简称ET)的超微结构研究仅国外有2例报道。我们对一例儿童ET患者治疗前和治疗后的血小板超微结构进行了观察,现报告如下。 材料和方法 一、患儿于治疗前取血一次,用马利兰治疗六个月后再取血一次,每次取血均以二例健康儿童作对照组。 二、制样方法中,取血时所用的玻璃器皿均事先进行硅化。抽取静脉血5毫升(3.8％枸椽酸钠抗凝),立即离心(500转/分钟)10分钟,制取血小板富有层(简称PRP),分三份进行如下处理:
At present, only two cases have been reported abroad in the ultrastructure study of children with essential thrombocythemia (referred to as ET). We observed a pediatric ET patients before and after treatment of platelet ultrastructure was observed, are as follows. Materials and methods First, the children take blood once before treatment, and then took the blood once every six days after the treatment with Malilan, each taking two healthy children as the control group. Second, the sample preparation method, the blood vessels used in glassware prior silicification. Venous blood was drawn 5 ml (3.8% sodium citrate anticoagulation), immediately centrifuged (500 r / min) for 10 minutes, prepared platelet rich layer (referred to as PRP), were treated in three copies as follows: