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采用液体菌种代替固体菌种栽培草菇,能缩短菌种周期,提高产量、质量,简化制种工艺,降低成本,提高劳动效率。一、材料 1.菌种:V～238。 2.培养基:a、摇瓶培养基:麦粉1％,白糖1％,葡萄糖1％,酵母粉1％,蛋白胨0.2％,KH_2PO_40.1％,MgSO_40.05％。pH值:灭菌前7.2,灭菌后7·0。500ml三角瓶每瓶装培养基100m1。灭菌:15磅30分钟。b.发酵培养基:麦粉2％,白糖1％,葡萄糖1％,蛋白胨0.3％,KH_2 PO_4 0.1％,MgSO_40.05％,豆油或花生油0.5％。pH
The use of liquid bacteria instead of solid fungus cultivation of mushroom, mushroom can shorten the cycle, increase yield and quality, simplify seed production process, reduce costs and improve labor efficiency. First, the material 1. Strain: V ~ 238. 2. Medium: a, shake flask medium: 1% of wheat flour, sugar 1%, glucose 1%, yeast 1%, peptone 0.2%, KH 2 PO 40.1%, MgSO 40.05%. pH: before sterilization 7.2, after sterilization 7 · 0.500ml flask bottled culture medium 100m1. Sterilization: 15 pounds for 30 minutes. b. Fermentation medium: wheat flour 2%, sugar 1%, glucose 1%, peptone 0.3%, KH 2 PO 4 0.1%, MgSO 40.05%, soybean oil or peanut oil 0.5%. pH