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目的：探讨常温下等离子体对高浓度乙肝病毒的灭活情况。方法：将乙肝疫苗、乙肝阳性血清和ＨＢＶ置于直流电源激发等离子体灭菌装置内，在等离子体环境下作用５ｍｉｎ或１０ｍｉｎ。通过固相放免法检测ＨＢｓＡｇ，电镜下观察病毒形态和ＨＢＶＤＮＡＰＣＲ法检测ＨＢＶＤＡＮ。结果：直流电源激发空气产生的等离子体，在常温下不能有效灭活ＨＢＶ。结论：等离子体是物理方法灭菌的最新技术，值得深入研究
Objective: To investigate the inactivation of plasma with high concentration of hepatitis B virus at room temperature. Methods: Hepatitis B vaccine, hepatitis B positive serum and HBV were placed in a DC power source-excited plasma sterilizing device, and were allowed to act for 5 minutes or 10 minutes in a plasma environment. HBsAg was detected by solid-phase radioimmunoassay. The virus morphology was observed under electron microscope and HBVDAN was detected by HBVDNA PCR. Results: The plasma generated by the DC power supply can not effectively inactivate HBV at room temperature. Conclusion: Plasma is the latest technology for sterilizing in physical ways and deserves further study