Three different treatment methods,including topical application method,injection method and immersion method were employed to treat 17α-hydroxyprogesterone,so as to investigating its effect on ovarian development red swamp crayfish( Procambarus clarkii). The results showed that all the three different methods could facilitate the synchronous ovarian development of red swamp crayfish at different degree,with the degree of maturity of 10% high compared with the control group. The highest maturity degree was 50% that was observed in immersion treatment group. The effect was determined as in order immersion method> injection method > topical application method > control group. The immersion method and the injection method could facilitate synchronization spawning of red swamp crayfish,with the order of immersion method 32. 5%,injection method 20%,topical application 17. 5% and the control group 17. 5%. Taking the synchronization spawning rate,final spawning rate and mortality into account,the order was the immersion method > injection method > topical application method > control group. Three different treatment methods, including topical application method, injection method and immersion method were employed to treat 17α-hydroxyprogesterone, so as to investigating its effect on ovarian development red swamp crayfish (Procambarus clarkii). The results showed that all the three different methods could facilitate the synchronous ovarian development of red swamp crayfish at different degree, with the degree of maturity of 10% high compared with the control group. The highest maturity degree was 50% that was observed in immersion treatment group. The effect was determined as in immersion method> injection method> topical application method> control group. The immersion method and the injection method could facilitate synchronization spawning of red swamp crayfish, with the order of immersion method 32. 5%, injection method 20%, topical application 17. 5 % and the control group 17. 5%. Taking the synchronization spawning rate, final spawning rate and mortality into account , the order was the immersion method> injection method> topical application method> control group.