论文部分内容阅读
为了探讨对大鼠下颌施加向后上方向的牵引力抑制髁头生长的机理,本实验用酶组织化学和透射电镜方法,研究了髁头软骨受力后的超微结构和代谢功能变化。结果发现,受力后髁头生发层细胞变为梭形,突起减少,线粒体明显肿胀,各种酶活性均下降,表明其形态及代谢功能均受干扰,可能与生发层缺乏基质的保护作用有关。成熟层细胞受力后,粗面内质网扩张,线粒体酶活性下降,乳酸脱氢酶和溶酶体酶活性升高,表明其能量供给受阻,使细胞内酸性粘多糖分泌功能下降。组织内氧及酸性粘多糖含量下降,促使钙磷离子进入基质结合,抑制髁头的分化功能。
In order to investigate the mechanism of exerting backward and upward traction on rat mandibular condyle to inhibit the growth of condylar head, ultrastructural and metabolic changes of condylar cartilage were studied by enzyme histochemistry and transmission electron microscopy. The results showed that the cells in the germinal layer of the condylar head became fusiform after the stress, the protrusions decreased and the mitochondria were obviously swollen, and the activity of various enzymes decreased, indicating that their morphology and metabolism were disturbed and may be related to the lack of protective effect of the germinal layer on the stroma . Mature layer of cells under stress, the rough endoplasmic reticulum dilated, mitochondrial enzyme activity decreased lactate dehydrogenase and lysosomal enzyme activity, indicating that the energy supply is blocked, so that the secretion of intracellular acidic mucopolysaccharide decreased. Tissue oxygen and acid mucopolysaccharide content decreased, prompting calcium and phosphorus ions into the matrix binding, inhibition of the differentiation of condylar head function.