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本文建立了高效液相色谱法测定人体内普罗帕酮血浆浓度。以YWG-C_(18)(10μm)为固定相,改性甲醇-乙腈-醋酸盐缓冲液(45.5:19.5:35)为流动相,用达克罗宁作内标在254nm波长处定量测定。方法最低检测限为5ng(S/W=3),血浆中最低检测浓度为25ng/ml,普罗帕酮血浆浓度在50～500ng/ml范围内线性关系良好,方法回收率为100.5％,不同浓度水平测定蛄果的日内和日间变异系数均小于3％。方法重现性好,专一性强,内源性物质不干扰,操作简便,快速,能适合梏床血药浓度监测及药代动力学研究。
This paper established a HPLC method for the determination of propafenone in human plasma. The mobile phase consisted of YWG-C 18 (10μm) as stationary phase and modified methanol-acetonitrile-acetate buffer (45.5: 19.5:35) as mobile phase. Quantitative determination was carried out at 254nm with dacronin as internal standard . The method has the lowest detection limit of 5ng (S / W = 3), the lowest detection concentration in plasma is 25ng / ml and the propanone plasma concentration is in the range of 50 ~ 500ng / ml. The method recovery is 100.5% The intra-and inter-day coefficient of variation (CV) of fruit level was less than 3%. The method has good reproducibility, specificity and does not interfere with the endogenous substances. The method is simple and rapid in operation and can be suitable for the monitoring of blood concentration and pharmacokinetic study in the gingival beds.