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目的 探讨增生性瘢痕、瘢痕疙瘩成纤维细胞中肌动蛋白 (actin)与瘢痕挛缩的关系。方法 取增生性瘢痕 10例 ,瘢痕疙瘩 5例 ,对成纤维细胞进行培养 ,并抽提细胞内蛋白成份后用 SDS- PAGE电泳 ,然后通过凝胶扫描仪测定两种组织来源的成纤维细胞内的总肌动蛋白、纤维状肌动蛋白 (F肌动蛋白 )、球形肌动蛋白 (G肌动蛋白 )的量 ,并计算 F/ G肌动蛋白的比值。结果 增生性瘢痕细胞每 10 4个细胞内含总肌动蛋白、F肌动蛋白、G肌动蛋白分别为 2 .38ng、0 .98ng、1.42 ng,F/ G肌动蛋白的比值为 0 .6 8,而瘢痕疙瘩分别为 1.6 8ng、0 .46 ng、1.2 6 ng及 0 .36。统计学分析 ,在总肌动蛋白、F肌动蛋白及 F/ G肌动蛋白的比值上两种瘢痕有非常显著性差异(P<0 .0 1) ,而 G肌动蛋白在两种瘢痕中无显著性差异 (P>0 .0 5 )。结论 瘢痕挛缩与细胞内总肌动蛋白、F肌动蛋白及 F/ G肌动蛋白的值密切相关 ,细胞内总肌动蛋白、F肌动蛋白及 F/ G肌动蛋白的比值不同可作为区分增生性瘢痕和瘢痕疙瘩的依据之一。
Objective To investigate the relationship between actin and scar contracture in hypertrophic scars and keloid fibroblasts. Methods 10 cases of hypertrophic scars and 5 cases of keloids were cultured, fibroblasts were cultured, protein components of the cells were extracted and analyzed by SDS-PAGE, and then the fibroblasts derived from the two tissues were determined by gel scanner Of total actin, F-actin (F-actin), globular actin (G-actin), and the F / G actin ratio was calculated. Results The percentage of total actin, F actin and G actin in proliferating scarring cells was 2.38 ng, 0.98 ng and 1.42 ng, respectively. The ratio of F / G actin was 0. 6 8, while keloids were 1.6 8 ng, 0.46 ng, 1.2 6 ng and 0.36, respectively. Statistical analysis showed that there was a significant difference between the two scars in the ratios of total actin, F actin and F / G actin (P <0.01), whereas G-actin showed significant differences in both scars There was no significant difference (P> 0.05). Conclusions The scar contracture is closely related to the total intracellular actin, F actin and F / G actin, and the difference of total actin, F actin and F / G actin in cells can be used as One of the bases to distinguish between hypertrophic scars and keloids.