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水稻直链淀粉含量直接影响了稻米的食味和加工品质,所以该含量性状已成为水稻品质育种研究的一个重要方向。目前关于水稻直链淀粉基因以及相关合成酶的研究报道虽很多,但可用于直链淀粉含量检测的标记的报道却很少。本研究选取不同直链淀粉含量的2个特异引物,用不同来源的302份水稻品种(系)进行检验分析,结果表明标记RMamc10-3能鉴定出约51%的低直链淀粉含量的水稻种质,标记Pul-t3鉴定高直链淀粉含量水稻种质的可靠性仅为38.9%。此外,发现云南的水稻品种(系)稻米的直链淀粉含量较低,有70%以上种质的稻米直链淀粉含量在5%~20%之间。在来自国际水稻所、缅甸和斯里兰卡的水稻种质中发现10份直链淀粉含量高于25%的材料。本研究得到的2个高和低直链淀粉含量标记,以及筛选获得的高和低直链淀粉含量的种质基因资源,都将为下一步开展水稻优质米研究和品质改良提供重要的材料。
The rice amylose content has a direct impact on the taste and processing quality of rice, so the content trait has become an important direction for rice quality breeding research. At present, there are many reports about the amylose gene and related synthetases in rice, but there are few reports on the markers that can be used for amylose content detection. In this study, two specific primers with different amylose content were selected and tested with 302 rice varieties (lines) from different origins. The results showed that the marker RMamc10-3 could identify about 51% rice varieties with low amylose content Quality and Mark Pul-t3 Identification of High Amylose Content The reliability of rice germplasm was only 38.9%. In addition, it was found that the amylose content of rice varieties in Yunnan was lower, and the amylose content in rice with more than 70% germplasm was between 5% and 20%. Ten rice materials with amylose content> 25% were found in rice germplasm from Iryo, Myanmar and Sri Lanka. The two high and low amylose content markers obtained from this study, as well as the high and low amylose content germplasm resources screened, will provide important materials for further research on rice quality and quality improvement.