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本文利用冠脉结扎/放松方法和Langendorff灌注技术,建立在体和离体大鼠心脏缺血/再灌注(ischemia/reperfusion,I/R)损伤模型,探讨白藜芦醇甙(polydatin)对大鼠I/R心肌损伤的保护作用及其机制。观察白藜芦醇甙对缺血和再灌注心律失常、心肌梗死面积、心脏收缩功能、心肌超氧化物歧化酶(superoxide dismutase,SOD)活性、丙二醛(malondialdehyde,MDA)含量、NO含量以及一氧化氮合酶(nitric oxide synthase,NOS)活性的影响。结果显示:与对照组相比,白藜芦醇甙组大鼠缺血和再灌注心律失常明显降低(P<0.05,P<0.01);心肌梗死面积显著减少(P<0.01);I/R心脏左心室发展压(left ventricular developedpressure,LVDP)、左心室压力上升和下降最大变化速率(±LVdp/dtmax)、冠脉流量(coronary flow,CF)明显改善(P<0.05,P<0.01);心肌SOD活性升高,MDA含量降低(P<0.05);NO含量和NOS及cNOS活性也明显升高(P<0.05);此外,NOS抑制剂L-NAME拮抗白藜芦醇甙对I/R心肌的保护作用。结果提示:白藜芦醇甙具有明显的抗心肌I/R损伤作用,此作用主要由cNOS产生的NO增加所介导。
In this study, we used the coronary ligation/relaxation method and Langendorff perfusion technique to establish a rat model of ischemic/reperfusion (I/R) injury in vitro and in vivo to investigate the effect of resveratrol on polydatin Myocardial Ischemic Injury and Its Mechanism in Rats Observed the effects of resveratrol on ischemia and reperfusion arrhythmia, myocardial infarct size, cardiac systolic function, myocardial superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and NO content. The effect of nitric oxide synthase (NOS) activity. The results showed that compared with the control group, ischemia and reperfusion arrhythmia were significantly decreased in the resveratrol-treated group (P<0.05, P<0.01); myocardial infarct size was significantly reduced (P<0.01); I/R Left ventricular developed pressure (LVDP), maximum rate of left ventricular pressure rise and fall (±LVdp/dtmax), and coronary flow (CF) were significantly improved (P<0.05, P<0.01). Myocardial SOD activity increased, MDA content decreased (P<0.05); NO content and NOS and cNOS activity also increased significantly (P<0.05); In addition, NOS inhibitor L-NAME antagonized resveratrol glycoside to I/R Myocardial protection. The results suggest that resveratrol has significant anti-myocardial I/R injury, which is mainly mediated by the increase of NO produced by cNOS.