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稻瘟病是我国水稻主产区的重要病害之一,其主效抗性基因Pi-ta和Pi-b在我国很多稻区表现广谱持久的稻瘟病抗性,被广泛应用于我国的水稻育种和生产。本研究选用稻瘟病抗性基因Pi-ta和Pi-b及其等位基因的功能标记,在对22份分别已知抗病基因Pita和Pi-b以及感病基因pi-ta与pi-b组成的水稻品种检测验证基础上,建立了2套稻瘟病基因多重PCR体系:体系I同时检测抗病基因Pi-ta与Pi-b,体系II同时检测感病基因pi-ta与pi-b,并利用2套体系对336份高世代育种材料进行检测,与单标记检测结果比较,表现稳定可靠,重复性好。本研究构建的抗稻瘟病基因分子标记多重PCR体系可用于水稻种质资源的快速评价和抗稻瘟病分子标记辅助育种。
Rice blast is one of the most important diseases in the main rice producing areas of our country. Its main resistance genes Pi-ta and Pi-b have broad-spectrum and long-lasting blast resistance in many rice areas in China and are widely used in rice breeding in China And production. In this study, we selected the functional markers Pi-ta and Pi-b and their alleles of rice blast resistance genes. In the study of 22 known resistance genes Pita and Pi-b and the susceptibility genes pi-ta and pi-b Two sets of rice blast disease gene multiplex PCR system were established on the basis of the detection and identification of rice varieties. The system I was used to detect Pi-ta and Pi-b, and the system II was used to detect the susceptible genes pi-ta and pi-b. Two sets of systems were used to detect 336 high-generation breeding materials. Compared with single-marker test results, the results showed stable and reliable, good reproducibility. The multiplex PCR system for blast resistance genes constructed in this study can be used for rapid evaluation of rice germplasm resources and molecular marker-assisted breeding for resistance to rice blast.