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目的:建立炒蔓荆子的含量测定方法及考察生品与清炒样品中蔓荆子黄素的含量变化。方法:采用高效液相色谱法,色谱柱为迪马kromasil(250 mm×4.6 mm,5μm),流动相为甲醇-0.4%磷酸溶液(60∶40),检测波长为258 nm,流速为1.0 mL/min,柱温为35℃。结果:对收集到的5个省的样品进行统一炮制,对比炮制前后蔓荆子黄素的含量变化,并对16个省(市)市售蔓荆子药材按照上述色谱条件进行了含量测定,不同地区的市售样品蔓荆子黄素的含量差异很大,含量最高的可达0.093%,最低为0.020%。结论:本方法简便、准确、重复性好,为制定蔓荆子的质量标准提供了依据。
OBJECTIVE: To establish a method to determine the content of Vitexin and to investigate the content change of Vitexin in raw and fried samples. Methods: The HPLC was performed on a Dima kromasil (250 mm × 4.6 mm, 5 μm) column with a methanol-0.4% phosphoric acid solution (60:40) at a detection wavelength of 258 nm and a flow rate of 1.0 mL / min, the column temperature is 35 ℃. Results: The collected samples from 5 provinces were processed in a unified way to compare the content of vinpocetin before and after processing. The contents of vinpocetin in 16 provinces (municipalities) were determined according to the above chromatographic conditions. Varieties of vinpocetin in different areas of the market samples vary widely in content, the highest content of up to 0.093%, the lowest 0.020%. Conclusion: The method is simple, accurate, reproducible, and provides the basis for the quality standard of Vitex.