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目的:探讨黄连阿胶汤对焦虑小鼠行为干预效果及其作用机制。方法:将实验小鼠随机分为4组,即空白对照组、地西泮组(DZP,1 mg.kg-1)、黄连阿胶汤低、高剂量组(2.5,5 g.kg-1),分别ig给予相应药物10 d。进行高架十字迷宫实验,记录动物进入开臂次数(OE)、进入封闭臂次数(CE)、进入开放臂时间(0T)和进入封闭臂时间(CT)等指标;高效液相色谱检测γ-氨基丁酸(GABA)。结果:黄连阿胶汤低、高剂量均能使小鼠OE+CE,OE%,OT%明显升高(P<0.01),其中高剂量组对OE%及OT%影响与DZP无显著性差异;黄连阿胶汤低、高剂量均能使小鼠血清GABA水平升高(P<0.01),其中高剂量组作用与DZP效果相当。结论:黄连阿胶汤有明显的抗小鼠焦虑作用,其作用机制可能与调节体内抑制性氨基酸GABA的水平有关。
Objective: To investigate the effect of huanglian gelatin soup on behavioral intervention in anxiety mice and its mechanism. Methods: The experimental mice were randomly divided into 4 groups: blank control group, diazepam group (1 mg.kg-1), huanglian gelatin low and high dose group (2.5 and 5 g.kg-1) , Respectively, given the appropriate drug ig 10d. The subjects were enrolled in the study. Elevated plus maze test was performed to record the number of OE, CE, 0T and CT; Butyric acid (GABA). Results: OE + CE, OE% and OT% of the mice treated with Huanglian gelatin capsules were significantly increased (P <0.01), and there was no significant difference between OE% and OT% and DZP in the high dose group. Huanglian gelatin soup low and high doses can make mice serum GABA levels (P <0.01), of which the high-dose group and DZP effect. Conclusion: Huanglian gelatin soup has obvious anti-mouse anxiety effect, and its mechanism may be related to the regulation of the level of inhibitory amino acid GABA in vivo.